Chlamydia trachomatis nucleic acid amplification test

This test detects bacterial DNA gene sequences in certain body fluids. This test is used when an infection with a bacteria called Chlamydia trachomatis is suspected in the urinary and genital organs. A sample of endocervical cells, urethral cells or discharge, or clean urine may be collected for this test.

Laboratory tests may be done for many reasons. Tests are performed for routine health screenings or if a disease or toxicity is suspected. Lab tests may be used to determine if a medical condition is improving or worsening. Lab tests may also be used to measure the success or failure of a medication or treatment plan. Lab tests may be ordered for professional or legal reasons. You may need this test if you have:

• Genitourinary chlamydia infection

Endocervical cells:

Written consent will be required for endocervical sampling. Review the consent form with the healthcare worker and ask any questions that you have before signing the consent form.

Tell the healthcare worker if you have a medical condition or are using a medication or supplement that causes excessive bleeding. If possible, schedule the procedure one week after your menstrual period. Do not douche or have sexual intercourse for 24 hours before the procedure.

You may be asked to urinate prior to your endocervical sampling. This will make it easier for the healthcare worker to see your cervical canal during the procedure and may make the procedure more comfortable for you.

Urethral cells/discharge:

Before collection of urethral cells and/or urethral discharge for this test, you should not urinate during the hour prior to the test. This is because urine may minimize or clear your urethra (the tube that drains urine) of any organisms (germs), thus affecting the results of this test.

Urinate an hour or more before a sample is collected.

Clean urine:

To prepare for giving a urine sample, be sure to drink enough fluids before the test, unless you have been given other instructions. Try not to empty your bladder before the test.

Urinate an hour or more before a sample is collected.

A sample of endocervical cells, urethral cells or discharge, or clean urine may be collected for this test.

Endocervical cells:

For an endocervical sampling, you will be asked to lie on your back with your legs spread and feet placed in stirrups. A speculum will be inserted into your vagina. This tool is used to gently spread apart your vagina. A small brush is inserted into the endocervical canal and rotated. This is done to collect cells. Once a sufficient sample is collected, the brush is removed. The sample off the brush is then sent for testing.

Urethral cells/discharge:

A urethral culture procedure is used to collect cells samples and/or urethral discharge. This test is usually done on males only. For this procedure, you will be asked to lie on your back. The tip of the penis will be cleaned. A special thin swab will be inserted into the urethra. The swab will gently be twisted side to side and then remain still for a few seconds before it is removed. This is to allow the swab to absorb enough fluid to be cultured. It may be possible to get a sample by “milking” the urethra for discharge. Ask the healthcare worker if this is an appropriate option.

Clean urine:

Immediately before a test that requires a clean urine sample, males must clean, rinse, and dry the head of the penis. Females must clean, rinse, and dry the urethral area. As you begin urinating, allow a small amount of urine to go into the toilet, and then stop the urine stream. Position the container to collect your urine, and urinate into the container. Remove the container when it has a sufficient amount of urine in it, and finish urinating into the toilet.

The urine sample may also be collected from a urinary catheter. If a catheter is present, the healthcare worker will collect urine from the catheter for testing. If there is no catheter present, a catheter may need to be inserted temporarily to collect the sample. The area will be cleaned, and a sterile catheter will be inserted into your urethra. Urine will drain from the catheter into a container for the test. When sufficient urine has been collected, the catheter will be removed. A special bag may be used to collect a urine sample from an infant.

The amount of discomfort you feel will depend on many factors, including your sensitivity to pain. Communicate how you are feeling with the person doing the procedure. Inform the person doing the procedure if you feel that you cannot continue with the procedure.

Endocervical cells:

You may feel mild discomfort, cramping, or pain during an endocervical sampling.

Urethral cells/discharge:

A urethral culture generally takes less than a minute. However, you may feel discomfort when the swab moves in the urethra.

Clean urine:

There is usually no pain experienced when a clean-catch urine sample is given or when the sample is taken from an existing urinary catheter. If an intermittent (temporary) catheter is used to obtain the sample, you may feel some discomfort.

Endocervical cells:

After endocervical sampling, you may experience some light spotting (mild, occasional bleeding from the vagina). Generally, there are no activity restrictions after this procedure.

Urethral cells/discharge:

After a sample has been collected, avoid all sexual activities until you receive your results and speak to your healthcare worker.

Clean urine:

After collecting a urine sample, close the container if it has a lid. Place the container where the healthcare worker asked you to put it. Clean your hands with soap and water.

You have the right to help plan your care. To help with this plan, you must learn about your health condition and how it may be treated. You can then discuss treatment options with your caregivers. Work with them to decide what care may be used to treat you. You always have the right to refuse treatment.