Detection of Influenza A virus subtype H1N1 using polymerase chain reaction technique

This test detects swine-origin influenza virus in secretions (fluid samples) from the nose, mouth, and throat. Swine-origin influenza is also called swine flu or H1N1 virus. This test is used to diagnose swine flu.

Laboratory tests may be done for many reasons. Tests are performed for routine health screenings or if a disease or toxicity is suspected. Lab tests may be used to determine if a medical condition is improving or worsening. Lab tests may also be used to measure the success or failure of a medication or treatment plan. Lab tests may be ordered for professional or legal reasons. You may need this test if you have:

• H1N1 Flu

There is no preparation needed for this test.

Fluid samples from your nose, throat, or mouth may be collected for this test. Caregivers should do this test as soon as possible if you begin to have signs or symptoms of swine flu.

A nasopharyngeal swab, aspirate, or wash is done to collect cell samples from the upper part of your nose and throat. For a nasopharyngeal swab, you will be asked to tilt your head back. The person doing the test will use a special kind of swab and insert it into one of your nostrils. The swab will be rotated gently and then remain still for a few seconds before it is removed. This is to allow the swab to collect a large enough sample to be tested. This process will be repeated in the second nostril. For an aspirate or wash, the healthcare worker will use a syringe to push a small amount of sterile saline into your nose, then either apply gentle suction (for the aspirate) or use gravity to collect the resulting fluid (saline and mucous) into a cup. The sample is then sent to the lab for testing.

The amount of discomfort you feel will depend on many factors, including your sensitivity to pain. Communicate how you are feeling with the person doing the test. Inform the person doing the test if you feel that you cannot continue with the test.

During a nasopharyngeal swab, aspirate, or wash, you may feel slight discomfort when the swab or fluid enters into the nostrils. You may gag or cough during the procedure.

There are no special instructions to follow after this test.

You have the right to help plan your care. To help with this plan, you must learn about your health condition and how it may be treated. You can then discuss treatment options with your caregivers. Work with them to decide what care may be used to treat you. You always have the right to refuse treatment.