Detection of Helicobacter pylori using polymerase chain reaction technique

This test detects a bacteria Helicobacter pylori (H. pylori) using a special technique called polymerase chain reaction (PCR). PCR detects gene sequences in bacterial DNA. This test is used when an infection with H. pylori is suspected. A sample of saliva, stool, or stomach lining cells through gastric endoscopy may be collected for this test. If you are having a gastric endoscopy, this test may be performed during your endoscopy. However, an endoscopy is generally not performed just to collect a sample for this test.

Laboratory tests may be done for many reasons. Tests are performed for routine health screenings or if a disease or toxicity is suspected. Lab tests may be used to determine if a medical condition is improving or worsening. Lab tests may also be used to measure the success or failure of a medication or treatment plan. Lab tests may be ordered for professional or legal reasons. You may need this test if you have:

• Helicobacter pylori gastrointestinal tract infection

Saliva:

There is no preparation needed for this test.

Stool:

Before giving a stool sample, tell the healthcare worker if you have diarrhea or are using antibiotics, barium, bismuth, oil, iron, magnesium, or medication to stop diarrhea.

Stomach lining cells:

An endoscopy is a procedure that requires your written consent. Review the consent form with the healthcare worker and ask any questions that you have before signing the consent form. Tell the healthcare worker if you have a history of nosebleeds, throat infections, chest pain, heart conditions, or a recent heart attack. Inform the healthcare worker if you have a medical condition or are using a medication or supplement that causes excessive bleeding. You should also report if you have a history of allergic or other reactions to local anesthetics.

To prepare for an endoscopy, you may be offered a mild sedative prior to the procedure to help you relax. To decrease pain, you will also receive a local anesthetic.

A sample of saliva, stool, or stomach lining cells through gastric endoscopy may be collected for this test.

Saliva:

Saliva samples may be collected by having you spit into a plastic tube several times or by using a swab. For the swab method you will need to open your mouth wide. The person doing the test will use a long, sterile cotton swab to wipe the inside of your cheeks. The swab may be rubbed several times to collect the sample. Do not close your mouth when the sample is being collected. After the sample has been collected, the swab will be taken out and tested.

Stool:

For a stool sample, you will be asked to have a bowel movement into a special container. Avoid adding urine, water, tissues, or toilet paper to the stool sample.

Stomach lining cells:

An endoscope is done with conscious sedation through an IV catheter. A local anesthesia will be sprayed into your mouth to prevent you from gagging or coughing when the endoscope is inserted. The endoscope, a flexible fiberoptic instrument that carries a tiny camera, is passed through your mouth. A device called a bite block may be used to protect your teeth. The endoscope then passes through your throat, esophagus (food tube), stomach, and duodenum. Air will be introduced through the endoscope to view the lining of the organs. Samples of cells or tissues may be collected through the endoscope. These samples are then sent to laboratory for testing.

The amount of discomfort you feel will depend on many factors, including your sensitivity to pain. Communicate how you are feeling with the person doing the test. Inform the person doing the test if you feel that you cannot continue with the test.

Saliva:

This test usually causes no discomfort.

Stool:

This test usually causes no discomfort.

Stomach lining cells:

Before the endoscopy, a local anesthesia is sprayed into your mouth. You may feel mild discomfort at the back of your throat. This will make your swallowing difficult. You will also receive medicine called conscious sedation, which puts you in a dream-like state where you should not feel pain or remember the procedure. However, you are still awake enough to move and respond to directions. During the procedure, you may feel mild discomfort when the endoscope enters your mouth. You may also feel the movement of the scope in your abdomen. After the procedure, you may have a sore throat or cough for several days.

Saliva:

There are no special instructions to follow after this test.

Stool:

After giving a stool sample in a healthcare facility, close the container if it has a lid, and place the container where the healthcare worker instructed. Clean your hands with soap and water. If you have been asked to collect the stool sample while at home, follow the directions provided.

Stomach lining cells:

Following an endoscopy and collection of a sample of cells or tissue, you will need to rest until healthcare workers say that you are able to leave the facility. Your throat may feel sore or dry for a short time. Contact your healthcare worker if you cough up significant amounts of bright red or dark-colored blood, have a high fever, have difficulty swallowing, or have black stools.

You have the right to help plan your care. To help with this plan, you must learn about your health condition and how it may be treated. You can then discuss treatment options with your caregivers. Work with them to decide what care may be used to treat you. You always have the right to refuse treatment.